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The next generation 4Pi microscope is here.  And it has adaptive optics.

Posted by Michael Feinberg on Tue, Jul 19, 2016 @ 04:02 PM

Tags: deformable mirror, adaptive optics, biological imaging, two photon, fluorescence, microscopy

Recently, work has been going on at Yale W-4PiSMSN Whole Cell 4Pi single marker switching nanoscopy graphical abstractUniversity, the Gurdon Institute at Cambridge University and Purdue University with funding provided by the Wellcome Trust Research Programme to develop a high resolution widefield microscope capable of imaging entire cell structures at once. This group has published an article in the journal Cell which describes the W-4PiSMSN (Whole Cell - 4Pi single marker switching nanoscopy).  In this article they show the results of imaging the endoplasmic reticulum (ER), bacteriophages, mitochondria, nuclear pore complexes, primary cilia, Golgi-apparatus-associated COPI vesicles, and mouse spermatocyte synaptonemal complexes.  The instrument includes two Boston Micromachines Multi Deformable Mirrors which are used to optimize the optical wavefront due to aberrations in both the instrument and biological sample.  We're so excited about this incredible breakthrough and I am sure you will be too!!!!

The full article, images and videos can be found here:



What You Missed this Month in the Adaptive Optics Industry

Posted by Angelica Perrone on Tue, Mar 31, 2015 @ 12:00 PM

Tags: deformable mirror, adaptive optics, biological imaging, BMC, imaging systems, two photon, microscopy, Mirrors, astronomy

If you've been hiding out in the lab, constantly checking your March Madness bracket, or escaping the cold to find any ounce of warmth (like me!), chances are you may have missed some exciting news. March turned out to be quite a busy month in the Adaptive Optics world, so here’s what you missed:

1. Exoplanet imaging in under a minute

At the recent AAAS 2015 annual meeting, Bruce Macintosh from Stanford University and Principal Investigator for the Gemini Planet Imager (GPI), discussed that there are over 1,000 confirmed planets due to the help of Adaptive Optics. In order to start really understanding a planet and its characteristics, you need to look at the composition of its atmosphere. Astronomers have relied heavily on the adaptive optics technique, allowing for distortion correction of the atmosphere using a deformable mirror. This has had proven success in regards to GPI, which uses a BMC deformable mirror (4K-DM), as part of its adaptive optics system. It can image planets in about a minute, which used to take up to an hour! GPI recently imaged the HR8799 star system, with three orbiting planets. Since November 2013, GPI has imaged 600 stars and identified 50-60 new planets.

2. 2nd quadruple star system discovered 

More exciting news from the Astronomy world was the discovery of a massive planet with a quadruple star system only 125 light-years from Earth. Discovered by the Jet Propulsion Laboratory, they were able to detect the fourth star after fitting the telescopes at the Palomar Observatory with a Robo-AO adaptive optics system. Utilizing the AO technique allowed astronomers to pick up on the faint star that couldn’t be seen before. Below is a diagram illustrating the discovered Ari 30 alongside its pair in a binary systems. Before this detection, only one other planet in a quadruple star system had been discovered before. More sightings are being predicted as exoplanets are found, with the help of Adaptive Optics systems of course!Ari 30 system resized 600

(Photo credit: NASA / JPL-Caltech)

3. The most valuable brains 

Congratulations to Scientists Winfried Denk, Arthur Konnerth, Karel Svoboda and David Tank for being awarded the world’s most valuable neuroscience prize, The Brain Prize, for the invention and development of two-photon microscopy! Two-photon microscopy is helping researchers to understand the human brain and how its networks process information, such as nerve cell communication. It has also enabled the study of nerve cells that control vision, hearing and movement. Recent work has been carried out to implement adaptive optics systems on two-photon microscope systems around the word, including locations such as the Howard Hughes Medical Institute, Institute Langevin at CNRS and Boston University. Standing on the shoulders of giants to improve imaging of the brain with AO!

Now that you are caught up with some of the biggest achievements using Adaptive Optics this past month, check out all of our deformable mirror products that are used in AO instruments like GPI on our website. Questions? Looking for a deformable mirror that will fit your needs? Contact BMC here



AO 101 Whitepaper

Looking to learn more about Adaptive Optics? Download our whitepaper to learn the fundamentals and how our customers are implemeting our DM's into their AO systems. 





Article links:

Telescope tech lets us Look directly at new worlds
Spotted: A planet 10 times the size of Jupiter with four suns
Two photon Microscopy inventors awarded the most valuable neuroscience prize 

New Results by Dr. Meng Cui at HHMI Using Segmented 492-DM

Posted by Angelica Perrone on Fri, Oct 17, 2014 @ 11:00 AM

Tags: deformable mirror, adaptive optics, boston micromachines, resolution, biological imaging, deep tissue microscopy, Howard Hughes Medical Institute, Janelia Farm Research Campus, SLM, spatial light modulator, BMC, imaging systems, two photon, fluorescence, segmented, microscopy

Segmented 492-DMDr. Meng Cui at the Howard Hughes Medical Center has recently pioneered Super Penetration Multi-Photon Microscopy (S-MPM) at the Cui Lab. He has successfully reported on focusing light through static and dynamic strongly scattering media using our segmented 492-DM (See more on the application here). By using the iterative multi-photon adaptive compensation technique (IMPACT), he since reported new results on in vivo fluorescence microscopy, providing a unique solution to noninvasive brain imaging. I HIGHLY encourage everyone to read his paper for in depth details of his technique here.

As of today, IMPACT has been the only technique used for in vivo microscopy. Due to the complicated wavefront distortion encountered in highly scattering biological tissue, IMPACT has the highest success rate in enabling neuron imaging through intact skulls of adult mice. Through Dr. Cui's testing, he has proven that even with the unpredictable motion of awake mice, IMPACT using the segmented 492-DM were able to perform wavefront measurements and improve the image quality.

Dr. Cui used the BMC segmented 492-DM as both the wavefront modulation and correction device. The IMPACT measurement works by splitting the DM’s pixels and running parallel phase modulation with each actuator at a unique frequency. Modulating only a portion of the pixels while keeping the rest stationary, a linear phase shift is then used as a function of time over the entire 2π phase range. The unique modulation frequency then becomes the unique phase slope value.  At the end of the modulation, a Fourier transform is used in IMPACT to determine the correction phase values. Dr. Cui then goes on to explain in detail how to determine what fraction of the pixels should be modulated, how to split the pixels into two evenly distributed groups and how the Nyquist-Shannon sampling theorem is integrated.

The imaging starts by setting the laser beam at the point of interest. The parallel phase modulation begins at one half at a time. As the measurement progresses, the laser focus becomes stronger, and laser power is gradually reduced to preserve the fluorescence signal from the sample. At the conclusion of the measurement, the compensated wavefront is displayed on the DM, and laser scanning in a conventional scope is begun. Figure 1 below shows the test setup for the experiment.

Meng Cui multiphoton microscopy test bed

Figure 1. Setup of the multiphoton microscope integrated with IMPACT. 

Dr. Cui used IMPACT for imaging the dendrites and spines of layer 5 pyramidal cells, in vivo at 650-670um under the dura in the mouse S1 cortex. In Media 1 below, you can see they are hardly resolvable with system correction only. In Media 2, you can see the dendrites and spines are clearly determined when full compensation has been applied.


Meng Video media 2Meng Video media 3Media 1. Hardly resovable dendrites and spines        Media 2. Resolved dendrites and spines


For the first time, IMPACT enabled in vivo two-photon fluorescence imaging through the intact skull of adult mice.  The technique also improved the fluorescence signal by a factor of ~20, along with overall resolution and contrast, this has proven to be a much greater adaptive optics imaging method than any other before. Dr. Cui also concluded that through these experiments, he found it worked well for awake, head-restrained animal imaging, providing a new and innovative solution for noninvasive studies of the mouse brain.

For more information on research going on at the Cui Lab, click here.

If you are interested in finding out more information on how the segmented 492-DM can help you achieve fluorescent imaging, please contact us here!

Adaptive Optics Correcting for Highly Scattering Media: A New Approach

Posted by Angelica Perrone on Thu, May 15, 2014 @ 12:05 PM

Tags: deformable mirror, adaptive optics, Boston University, deep tissue microscopy, Howard Hughes Medical Institute, SLM, spatial light modulator, BMC, imaging systems, two photon, fluorescence

Scattering media can be a real headache if you are looking to achieve high-resolution, deep tissue in vivo images. Without adaptive optics, do not anticipate having the optical control you need to correct for scattering media effectively. But no need to worry, we have a solution.test bed S MPM resized 600

Since standard Multiphoton Microscopy just wasn’t cutting it, the Cui Lab at Howard Hughes Medical Center pioneered a new technique that Boston University also recently developed, called Superpentation Multi-Photon Microscopy (S-MPM). Each group uses a different optimization scheme but the outcome is the same: The enhanced technique permits active compensation of wavefront aberrations in a scanning beam path through the use of a BMC MEMS Spatial Light Modulator (SLM), allowing for increased depth imaging.

Developed at Boston University and commercialized by Boston Micromachines, the enabling components are the Kilo-SLM and the high speed S-driver. With these components incorporated into the test bed shown in Fig. 1, images of 1 µm diameter fluorescent beads through 280 µm thick mouse skull can be achieved at depths of about 500 µm. The SLM corrected low order spherical aberrations as well as higher order scattering effects. Signal enhancement with higher resolution and contrast were improved by 10x-100x. The optimized SLM phase improves imaging over a field of view of 10-20 µm for samples tested to date with techniques currently in the works to improve upon this.

With 600 nm of stroke and 60 kHz of maximum frame rate, the Kilo-S System comes in a variety of options to fit your needs at a much reduced cost over our standard 1000 channel system. Contact us today for more information on our Kilo-S or any of our other systems! 

Photonics West/BiOS Exhibition Recap

Posted by Angelica Perrone on Wed, Feb 26, 2014 @ 11:45 AM

Tags: deformable mirror, adaptive optics, boston micromachines, laser beam, deep tissue microscopy, SLM, spatial light modulator, BMC, imaging systems, two photon, retinal imaging, free-space communication, modulating retroreflector, segmented, SPIE, Photonics West, microscopy, optical chopper, optical modulator, chopper, Adaptive Optics Scanning Laser Ohphthalmoscope, Joslin Diabetes Center, Mirrors

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Just a few weeks ago we arrived back from the Photonics West 2014 exhibition and conference in San Francisco, CA. I wanted to share details and further observations on the show for those present at the show and those not being able to attend this year. 

For the first time we made the decision to also attend the BiOS exhibition for the few days prior to PWest. Not being quite sure what to expect for booth traffic, especially since it conflicted with the superbowl, we still generated a good amount of interest for the smaller show. Our main presentations focused on our new adaptive optics-enhanced scanning laser ophthamoscope (AOSLO), the Apaeros Retinal Imaging System, which includes our Multi-DM, and the Superpenetration Multiphoton Microscopy technique, which is enabled by our Kilo-SLM and high speed S-Driver. Although both exhibits generated respectable notice and positive feedback, most people were familiar with the Superpentration Multiphoton work being done. Either wanting to try two-photon microscopy themselves or already in the process of doing so, our Kilo-SLM paired with our high speed S-driver presented data that was intriguing to most.

After wrapping up BiOS, we headed to the opposite side of the South hall at the Moscone Center for a larger booth setup for PWest. Here we had our entire mirror family on display, as well as live demonstrations of the Reflective Optical Chopper and Wavefront Sensorless Adaptive Optics Demonstrator for Beam Shaping (WSAOD-B). For this part of the exhibition, I would say our deformable mirrors produced the most attention, most likely due to our wide assortment of shapes and actuator counts up to 4092. The WSAOD-B live demonstration did generate a great deal of attention, as most people are unaware of how sensorless AO works. Besides our deformable mirror line, I would still say the Multiphoton Microscopy overview was initiating even further interest here as well.

Overall BMC had a great show and it seemed well worth it to expand our exhibit onto BiOS beforehand. Although this was my first time attending the show, I noticed every inch of space at PWest being used for exhibitor tables and booths, even setting up in front of the bathrooms! I hope to see PWest advance even larger, maybe one day expanding to its third space, West Hall. I look forward to next year’s show and hope to reconnect with you all again throughout the year.

If you were not able to attend the show and would like any information on the products mentioned, please visit our website and download our whitepapers

Further focus at CLEO 2013

Posted by Michael Feinberg on Tue, Jul 09, 2013 @ 12:54 PM

Tags: deformable mirror, adaptive optics, boston micromachines, laser beam, laser science, biological imaging, deep tissue microscopy, BMC, two photon, free-space communication, modulating retroreflector, optical chopper, optical modulator, chopper, UAV, pulse, pulse width, laser pulse shaping, ultrafast lasers, CLEO, AOM, acousto-optic modulator, speed, shutter

It's been a few weeks since we returned frocleo resized 600m the Conference on Lasers and Electro-Optics 2013 and now that we're settled back in to the daily routine, I thought I would give some highlights on the show. I was happy to be joined this time by our new Marketing and Communications Specialist, Angelica Perrone, who did a great job navigating the complex photonics market for the first time.

While the conference seems to be chugging along at a nice pace, the tradeshow has most definitely become a smaller venue.  We were once again hosted by our strategic partner, Thorlabs (thanks, again guys!) and being in such a central location on the floor, we were able to get a good flavor for the pace of the show.  Here are my thoughts:

Little, different, yellow, better

Anybody get that Nuprin reference?  Anybody? See what I 'm talking about here.

Okay, so it's not yellow (although yellow lasers are cool), but the show is definitely getting smaller.  I mentioned to a colleague that since the show is in San Jose for the second year in the row, it seemed like the barriers on either end of the tradeshow floor had moved in just a bit. 

As far as different, the show is not like other photonics shows in that it is pretty focused in its applications.  While there were some interesting talks on microscopy, this was a small portion of the material, with most others focussing on more laser-centric applications, as the title of the conference implies. 

As far as better, I would say that for BMC, it was most definitely better for our new products:  The Reflective Optical Chopper(ROC) and the Linear Array DM.  We recieved more interest in these products over our legacy deformable mirror technologies. This is exciting for me as a product marketer and salesperson and even moreso as a member of a company that is always looking for new avenues for our technology. We see the ROC being useful for users who span from pure laser scientists to imaging engineers interested in chopping a beam at high speed with either a constant or variable duty cycle.  The linear array has already proven useful in pulse shaping applications as described in our whitepaper, which is available for download here.  Both products are available for purchase now.

Our Wavefront Sensorless Adaptive Optics Demonstrator for Beam Shaping (WSAOD-B)also generated some buzz. More and more applications which require wavefront correction are surfacing and need a solution without a wavefront sensor.

In all, it was a good show that has given me and my team work to do as we explore more exotic applications for our technology.  I look forward to joining the show again next year and I hope to connect with all of you again in the near future!

For more information on the products mentioned above, please visit our website and download our whitepapers.

Improved Two Photon-Imaging Through Laser Pulse Compression with the Linear Array DM

Posted by Michael Feinberg on Fri, Dec 07, 2012 @ 09:05 AM

Tags: boston micromachines, Howard Hughes Medical Institute, Janelia Farm Research Campus, BMC, two photon, fluorescence, microscopy, laser pulse compression

In our last blog post (Fast and Precise Laser Pulse Compression with the Linear Array DM) we discussed research being done in the Cui Lab at HHMI’s Janelia Farm Research Campus that used our Linear Array DM for laser pulse compression.  In part two we examine a two photon fluorescence microscopy project led by associate Reto Fiolka at Janelia Farm that illustrates the use of the Linear Array’s potential as a pulse compressor for imaging applications using the phase resolved interferometric spectral modulation (PRISM) optimization technique.

The Linear Array pulse compressor setup was used to restore the laser pulse to its transform limited state, thus improving the ability to excite fluorescence by two photon absorption. A sample consisting of 10 micron diameter fluorescence beads (emission: 465 nm) was prepared and spread on a cover-slip. The laser beam first propagated through the pulse compressor and was subsequently focused on the sample using a 20X NA 0.5 Nikon objective. A 2D image was obtained by translating a motorized sample stage. Without spectral pulse shaping, only a weak fluorescence signal could be obtained (See figures a and c). Since the objective adds significant additional dispersion to the laser pulse, the spectral phase correction that had been determined previously using the photodiode could not be used. Therefore PRISM optimization was repeated using the fluorescence signal coming from the beads itself as a feedback signal.

Janelia Farm’s results show a dramatic increase in fluorescence signal for the optimized spectral phase (see figures b and d). The signal strength was increased by a factor of ~6.5

twophoton microscopy resized 600


According to Fiolka, “The tested device represents a promising alternative to liquid crystal displays, since the MEMS technology enables high filling factor, high efficiency and operation speed, exceptional phase stability and accuracy and can be used over a very broad wavelength spectrum.”

We're very excited about these results and we are currently working with other groups interested in reproducing these results on tissue samples.  Thanks again to Dr. Fiolka and the Janelia Farm group for their efforts in improving two photon imaging techniques!!

More details can be found in our Linear Array white paper which includes an application overview of this exciting project.  You can also link to the research directly using the links to the Cui Lab and the scientific publication above.