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The next generation 4Pi microscope is here.  And it has adaptive optics.

Posted by Michael Feinberg on Tue, Jul 19, 2016 @ 04:02 PM

Recently, work has been going on at Yale W-4PiSMSN Whole Cell 4Pi single marker switching nanoscopy graphical abstractUniversity, the Gurdon Institute at Cambridge University and Purdue University with funding provided by the Wellcome Trust Research Programme to develop a high resolution widefield microscope capable of imaging entire cell structures at once. This group has published an article in the journal Cell which describes the W-4PiSMSN (Whole Cell - 4Pi single marker switching nanoscopy).  In this article they show the results of imaging the endoplasmic reticulum (ER), bacteriophages, mitochondria, nuclear pore complexes, primary cilia, Golgi-apparatus-associated COPI vesicles, and mouse spermatocyte synaptonemal complexes.  The instrument includes two Boston Micromachines Multi Deformable Mirrors which are used to optimize the optical wavefront due to aberrations in both the instrument and biological sample.  We're so excited about this incredible breakthrough and I am sure you will be too!!!!

The full article, images and videos can be found here:



Tags: deformable mirror, adaptive optics, biological imaging, two photon, fluorescence, microscopy